Protocols for phytoplankton metabarcoding

• Sampling protocols 
  • Lake plankton sample collection from the field for downstream molecular analysis, Isabelle Domaizon,Rainer Kurmayer,Camilla Capelli,Cécile Chardon,Peter Hufnagl,Marine Vautier,Nico Salmaso : https://www.protocols.io/view/lake-plankton-sample-collection-from-the-field-for-xn6fmhe/metadata
  • Video prélèvement (A. Canino) https://youtu.be/x_t0-063Qno
• Plankton DNA extraction from Sterivex filter units
  • https://www.protocols.io/view/plankton-dna-extraction-from-sterivex-filter-units-bvgzn3x6
• Primers to use:
  • Canino et al. 2023. Which barcode to decipher freshwater microalgal assemblages? Tests on mock communities. Int J Limnol
  • https://doi.org/10.1051/limn/2023008
• Protocols in French :
  • Canino, A., Alric, B., Bouchez, A., Domaizon, I., Duran, C., LAPLACE-TREYTURE, C., & Rimet, F. (2025). Protocoles d’échantillonnage, extraction d’ADN et amplification de barcode du phytoplancton en vue d’un séquençage métabarcoding—PhytoDOM. https://zenodo.org/records/16942527

If you are interested in the classical phytoplankton protocols (microscope identification and quantification), you can access them here:

Rimet F. (2012). Protocoles d'analyse du phytoplancton de l'INRA : Prélèvement, dénombrement et biovolumes. Umr Carrtel, USMB, INRAE https://hal.science/hal-05523545